Developed by Charles G. Fleming and Thomas E. Goliber (cfleming@alleg.edu, tgoliber@alleg.edu), Allegheny College, Meadville, PA 16335.
Discipline: Biology
This program is a simulation of an experimental procedure, known as the interrupted mating technique, that can be used to determine the relative locations of genes on a bacterial genome. The procedure takes advantage of the bacterial gene exchange process known as conjugation. Bacterial conjugation takes place when two cells, a donor and a recipient, physically join together and the donor cell then transfers some of its genes to the recipient cell.
This computer simulation will simulate some of the basic steps involved in performing a gene mapping experiment using the interrupted mating technique, and generates data that can be plotted and used to map part of a bacterial genome.
The design of this program was based on mapping experiments done previously using E. coli. This computer E. coli has a total of 10 alleles (in addition to the streptomycin sensitive or resistant alleles).
This application is used for Introductory Biology (Molecules, Cells, and Genetics) at Allegheny College.
This application was developed under NeXTstep release 2.0.
